Collaboratore TER
Dipartimento Infrastrutture di ricerca per le risorse biologiche marine
Centro Microscopia Avanzata

Tel. +39 081 5833257 - +39 081 5833407 - +39 081 5833416 - +39 081 5833289
e-mail giovanni.gragnaniello(at)szn.it

Breve CV

1983 - 1988: Corso di addestramento in microscopia elettronica SZN.
1988: training in microscopia elettronica all’Università di Ulm, Germania.
1989 – 1999: Servizio microscopia elettronica SZN.
1999 - 2014: Bioimaging nel gruppo della dott.essa Santella.
dal 2015: Unità AMOBIO – sez. RIMAR – SZN.

Attività/Funzioni

Gestione operativa dei sistemi di bioimaging.
Analisi di immagine relativa agli esperimenti di bioimaging.

Journal Papers

Vasilev F, Chun JT, Gragnaniello G, Garante E, Santella L. Effects of ionomycin on egg activation and early development in starfish. PLoS One. 2012;7(6):e39231. doi: 10.1371/journal.pone.0039231. Epub 2012 Jun 18. PubMed PMID: 22723970; PubMed Central PMCID: PMC3377674.

Chun JT, Puppo A, Vasilev F, Gragnaniello G, Garante E, Santella L. The biphasic increase of PIP2 in the fertilized eggs of starfish: new roles in actin polymerization and Ca2+ signaling. PLoS One. 2010 Nov 23;5(11):e14100. doi: 10.1371/journal.pone.0014100. PubMed PMID: 21124897; PubMed Central PMCID: PMC2990714.

Kyozuka K, Chun JT, Puppo A, Gragnaniello G, Garante E, Santella L. Guanine nucleotides in the meiotic maturation of starfish oocytes: regulation of the actin cytoskeleton and of Ca(2+) signaling. PLoS One. 2009 Jul 20;4(7):e6296. doi: 10.1371/journal.pone.0006296. PubMed PMID: 19617909; PubMed Central PMCID: PMC2706993.

Monaco S, Illario M, Rusciano MR, Gragnaniello G, Di Spigna G, Leggiero E, Pastore L, Fenzi G, Rossi G, Vitale M. Insulin stimulates fibroblast proliferation through calcium-calmodulin-dependent kinase II. Cell Cycle. 2009 Jul 1;8(13):2024-30. Epub 2009 Jul 21. PubMed PMID: 19502797.

Puppo A, Chun JT, Gragnaniello G, Garante E, Santella L. Alteration of the cortical actin cytoskeleton deregulates Ca2+ signaling, monospermic fertilization, and sperm entry. PLoS One. 2008;3(10):e3588. doi: 10.1371/journal.pone.0003588. Epub 2008 Oct 30. PubMed PMID: 18974786; PubMed Central PMCID: PMC2570615.

Kyozuka K, Chun JT, Puppo A, Gragnaniello G, Garante E, Santella L. Actin cytoskeleton modulates calcium signaling during maturation of starfish oocytes. Dev Biol. 2008 Aug 15;320(2):426-35. doi: 10.1016/j.ydbio.2008.05.549. Epub 2008 Jun 6. PubMed PMID: 18599031.

Nusco GA, Chun JT, Ercolano E, Lim D, Gragnaniello G, Kyozuka K, Santella L. Modulation of calcium signalling by the actin-binding protein cofilin. Biochem Biophys Res Commun. 2006 Sep 15;348(1):109-14. Epub 2006 Jul 17. PubMed PMID: 16875665.

Moccia F, Nusco GA, Lim D, Ercolano E, Gragnaniello G, Brown ER, Santella L. Ca2+ signalling and membrane current activated by cADPr in starfish oocytes. Pflugers Arch. 2003 Aug;446(5):541-52. Epub 2003 May 16. PubMed PMID: 12756567.

Sabała P, Targos B, Caravelli A, Czajkowski R, Lim D, Gragnaniello G, Santella L, Barańska J. Role of the actin cytoskeleton in store-mediated calcium entry in glioma C6 cells. Biochem Biophys Res Commun. 2002 Aug 16;296(2):484-91. PubMed PMID: 12163045.

Lim D, Kyozuka K, Gragnaniello G, Carafoli E, Santella L. NAADP+ initiates the Ca2+ response during fertilization of starfish oocytes. FASEB J. 2001 Oct;15(12):2257-67. PubMed PMID: 11641253.

Tosti E, Di Cosmo A, Cuomo A, Di Cristo C, Gragnaniello G. Progesterone induces activation in Octopus vulgaris spermatozoa. Mol Reprod Dev. 2001 May;59(1):97-105. PubMed PMID: 11335951.

Santella L, De Riso L, Gragnaniello G, Kyozuka K. Cortical granule translocation during maturation of starfish oocytes requires cytoskeletal rearrangement triggered by InsP3-mediated Ca2+ release. Exp Cell Res. 1999 May 1;248(2):567-74. PubMed PMID: 10222148.

Santella L, De Riso L, Gragnaniello G, Kyozuka K. Separate activation of the cytoplasmic and nuclear calcium pools in maturing starfish oocytes. Biochem Biophys Res Commun. 1998 Nov 9;252(1):1-4. PubMed PMID: 9813135.

Dale B, Iaccarino M, Fortunato A, Gragnaniello G, Kyozuka K, Tosti E. A morphological and functional study of fusibility in round-headed spermatozoa in the human. Fertil Steril. 1994 Feb;61(2):336-40. PubMed PMID: 8299793.