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foto de PascaleSenior Technologist
Marine Biotechnology Department

Tel.: +39 
Fax: +39 
E-mail: donatella.depascale(at)szn.it
Contatto Skype: donatella.de.pascale

Curriculum Vitae

 


Research Interests

The research activity of Dr. Donatella de Pascale is mainly focused on the exploration of extreme marine environments such as the Antarctic, the Arctic and the Earth like Tibetan glaciers, in order to isolate and characterize new strains of bacteria and fungi hyper-producers of bioactive compounds such as antimicrobials and anti-biofilm and anti-cancer.
A classic biodiscovery pipeline has been implemented for the identification of new bioactive compounds, starting from the collection of sediments from extreme marine environments and the isolation of bacteria and fungi adapted to the purification and their structural elucidation. Among the selected targets, the aim is to discover new antimicrobials aimed at multi-resistant human pathogens.
New anthelmintic compounds are also selected using Caenorhabditis elegans as a model system, and tests are available for anti-biofilm and anti-cancer activity. The research activity is also aimed at the biochemical characterization of small antimicrobial peptides and at the development of new methods for the isolation of microorganisms in order to improve the isolation of bacteria belonging to the so-called "non-cultivable fraction of microorganisms".
The exploitation of bacteria for biotechnological purposes is also aimed at the discovery of new biocatalysts, in fact antifreeze proteins and hydrolases, which have a high potential for biotechnological applications are widely investigated. These enzymes are isolated using both the genomic and functional metagenomics approaches, using plate-based screening.

Selected Publications

Parrilli E, Tedesco P, Fondi M, Tutino ML, Lo Giudice A, de Pascale D, Fani R. The art of adapting to extreme environments: The model system Pseudoalteromonas. Phys Life Rev. 2019 Apr 4. pii: S1571-0645(19)30066-1. doi: 10.1016/j.plrev.2019.04.003.

Tortorella E, Tedesco P, Palma Esposito F, January GG, Fani R, Jaspars M, de Pascale D. Antibiotics from Deep-Sea Microorganisms: Current Discoveries and Perspectives. Mar Drugs. 2018 Sep 29;16(10). pii: E355. doi: 10.3390/md16100355. Review.

Tedesco P, Maida I, Palma Esposito F, Tortorella E, Subko K, Ezeofor CC, Zhang Y, Tabudravu J, Jaspars M, Fani R and de Pascale D. Antimicrobial activity of monoramnholipids produced by bacterial strains isolated from Ross sea (Antarctica). Mar Drugs. 2016 Apr 26;14(5). pii: E83. doi: 10.3390/md14050083.

Sannino F, Sansone C, Galasso C, Kildgaard S, Tedesco P, Fani R, Marino G, de Pascale D, Ianora A, Parrilli E, Larsen TO, Romano G, Tutino ML. Pseudoalteromonas haloplanktis TAC125 produces 4-hydroxybenzoic acid that induces pyroptosis in human A459 lung adenocarcinoma cells. Scientific Report 2018 Jan 19;8(1):1190. doi: 10.1038/s41598-018-19536-2.

Bosi E, Fondi M, Orlandini V, Perrin E, Maida I, de Pascale D, Tutino ML, Parrilli E, Lo Giudice A, Filloux A, Fani R. The pangenome of (Antarctic) Pseudoalteromonas bacteria: evolutionary and functional insights. BMC Genomics. 2017 Jan 17;18(1):93. doi: 10.1186/s12864-016-3382-y.

Tedesco P, Visone M, Parrilli E, Tutino ML, Perrin E, Maida I, Fani R, Ballestriero F, Santos R, Pinilla C, Di Schiavi E, Tegos G, de Pascale D. Investigating the Role of the Host Multidrug Resistance Associated Protein Transporter Family in Burkholderia cepacia Complex Pathogenicity Using a Caenorhabditis elegans Infection Model. PLoS One. 2015 Nov 20;10(11):e0142883. doi: 10.1371/journal.pone.0142883

de Pascale D, De Santi C, Fu J, Landfald B. The microbial diversity of Polar environments is a fertile ground for bioprospecting. Marine Genomics. 2012 Dec; 8:15-22.

Fu J, Leiros HK, de Pascale D, Johnson KA, Blencke HM, Landfald B. Functional and structural studies of a novel cold-adapted esterase from an Arctic intertidal metagenomic library. Appl Microbiol Biotechnol. 2012 May;97(9):3965-78.

De Santi C, Tutino ML, Mandrich L, Giuliani M, Parrilli E, Del Vecchio P, de Pascale D. The hormone-sensitive lipase from Psychrobacter sp. TA144: New insight in the structural/functional characterization. 2010 Biochimie. Aug;92(8):949-57

Tutino ML, di Prisco G, Marino G, de Pascale D. Cold-adapted esterases and lipases: from fundamentals to application. 2009. Protein Pept Lett. 16(10); 1172-1180.

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